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Determination of Kd and kpol for dGTP incorporation (A) and DXG-TP incorporation (B) into a DNA-RNA 30/45-mer primer-template. The polymerase rates were plotted against dGTP or DXG-TP concentrations, and the data were fit to a hyperbola to give a Kd of 12 ± 2 μM and a kpol of 34 ± 2 s−1 for dGTP and a Kd of 8.8 ± 1.1 μM and a kpol of 1.5 ± 0.1 s−1 for DXG-TP.
