TABLE 3.
Effects of DAPD and DXG on cell proliferation and mitochondrial function and morphology in HepG2 cells
| Compound | Concn (μM) | Cell no. (104/ml) | l-Lactate density (mg/106 cells) | % of mitochondrial DNA content to that for control | Lipid droplet formation | Mitochondrial morphology |
|---|---|---|---|---|---|---|
| Control | 0 | 16.0 ± 0.9a | 2.4 ± 0.2a | 100 | Negative | Normal |
| DAPD | 0.1 | 16.2 ± 0.4 | 2.3 ± 0.1 | 104 ± 10a | NDb | ND |
| DAPD | 1.0 | 15.9 ± 0.8 | 2.3 ± 0.2 | 100 ± 11 | ND | ND |
| DAPD | 10.0 | 13.2 ± 0.8 | 3.0 ± 0.1 | 92 ± 5 | Negative | Normal |
| Control | 0 | 25.2 ± 0.8 | 2.35 ± 0.02 | 100 | Negative | Normal |
| DXG | 0.1 | 26.1 ± 0.8 | 2.28 ± 0.20 | 106 ± 5 | ND | ND |
| DXG | 1.0 | 25.2 ± 0.6 | 2.45 ± 0.08 | 107 ± 8 | ND | ND |
| DXG | 10.0 | 24.9 ± 0.8 | 2.36 ± 0.10 | 99 ± 9 | Negative | Normal |
| Controlc | 0 | 42.9 ± 0.2 | 1.52 ± 0.04 | 100 | ND | ND |
| DAPDc | 25.0 | ND | ND | 64 ± 4 | ND | ND |
| DAPD | 50.0 | 45.7 ± 0.1 | 1.40 ± 0.13 | 36 ± 6 | ND | ND |
| DXGc | 25.0 | ND | ND | 103 ± 11 | ND | ND |
| DXG | 50.0 | 45.5 ± 0.2 | 1.62 ± 0.06 | 98 ± 3 | ND | ND |
a
Data are the means ± standard deviations of three measurements.
b
ND, not determined.
c
The second experiment was performed with higher concentrations of DAPD and DXG.