. 2001 Jan;45(1):158–165. doi: 10.1128/AAC.45.1.158-165.2001

TABLE 7.

Steady-state kinetic constants for dGTP and DXG-TP for HIV-1 RT and human DNA polymerases

Polymerase	K_m (μM) for dGTP^a	K_i (μM) for DXG-TP^a	K_i/K_m
HIV-1 RT^b	1.2 ± 0.4	0.019 ± 0.002	0.016
HIV-1 RT^c	0.6 ± 0.2	0.73 ± 0.17	1.2
DNA polymerase α^d	1.7 ± 0.7	78 ± 17	45.9
DNA polymerase β^d	2.3 ± 0.5	32 ± 2	13.9
DNA polymerase γ^d	3.0 ± 0.4	4.3 ± 0.4	1.4

Data are the means ± standard deviations of four measurements.

Poly(dC) · oligo(dG)_12–18 was used as the template-primer.

Heteropolymeric 23/44 primer-template used in the assay.

Activated calf thymus DNA was used as the template-primer.