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Pre-steady-state kinetic constants for dGTP and DXG-TP incorporation into D30/D45 and D30/R45 primer-templates
| Primer-template | dGTP analog | kpol (s−1) | Kd (μM) | kss (s−1) | kpol/Kd (μM−1 s−1) |
|---|---|---|---|---|---|
| D30/D45 | dGTP | 16 ± 1a | 6.1 ± 0.9 | 0.36 ± 0.03 | 2.6 |
| DXG-TP | 1.46 ± 0.03 | 2.5 ± 0.2 | 0.28 ± 0.09 | 0.58 | |
| D30/R45 | dGTP | 34 ± 2 | 12 ± 2 | 0.15 ± 0.06 | 2.8 |
| DXG-TP | 1.5 ± 0.1 | 8.8 ± 1.1 | 0.14 ± 0.03 | 0.17 |
Values are means ± standard errors.
