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. 2022 Apr 16;23(8):4410. doi: 10.3390/ijms23084410

Figure 3.

Figure 3

Transcriptional silencing of the mutant allele in patient-derived fibroblasts. Measurement of mRNA levels of the mutant and wild-type alleles of COL6A1 by allele-specific digital-droplet PCR in individuals’ fibroblasts. (A) Ratio of the expression of mutant versus wild-type alleles in fibroblasts untreated or edited with crRNA1 or crRNA2. (B) Normalized expression of the mutant allele in the edited fibroblasts. (C) Normalized expression of the wild-type allele in the edited fibroblasts. Data are presented as mean ± SD (n = 3). Student’s t test was used to compare the data (* p < 0.05; ** p < 0.01; *** p < 0.001).