. 2022 Apr 13;11(4):516. doi: 10.3390/antibiotics11040516

Table 4.

Primer sequences of genes of E. coli, P. aeruginosa and MRSA.

Organisms	Genes	Sequences of Primer (5′-3′)	Annealing	Reference
E. coli	fimH	F-TGCAGAACGGATAAGCCGTGG	60 °C for 1 min	[5]
	fimH	R-GCAGTCACCTGCCCTCCGGTA	60 °C for 1 min	[5]
	usp	F-ACATTCACGGCAAGCCTCAG	58 °C for 1 min	[5]
	usp	R-AGCGAGTTCCTGGTGAAAGC	58 °C for 1 min	[5]
P. aeruginosa	IMP	F-GAAGGCGTTTATGTTCATAC	55 °C for 1 min	[1]
	IMP	R-GTATGTTTCAAGAGTGATGC	55 °C for 1 min	[1]
	AIM	F-CTGAAGGTGTACGGAAACAC	54 °C for 1 min	[1]
	AIM	R-GTTCGGCCACCTCGAATTG	54 °C for 1 min	[1]
S. aureus	nuc	F-GCGATTGATGGTGATACGGTT	50 °C for 30 s	[34]
	nuc	R-AGCCAAGCCTTGACGAACTAAAG	50 °C for 30 s	[34]
	mecA	F-GATCGCAACGTTCAATTTAATTT	50 °C for 30 s	[34]
	mecA	R-GCTTTGGTCTTTCTGCATTCCT	50 °C for 30 s	[34]