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. 2022 Apr 7;11(4):728. doi: 10.3390/antiox11040728

Figure 4.

Figure 4

HSYA protected ZO-1 from oxidative damage: (A) protein expression of ZO-1, occludin, and claudin 5; (B) gene expression of ZO-1 (Tjp1), occludin (Ocln), and claudin 5 (Cldn 5); (C) ZO-1 protein degradation at the indicated time was determined when protein synthesis was inhibited by cycloheximide in LPS-stimulated bEnd.3 cells; (D–F) immunofluorescence showed the expression of ZO-1, occludin, and claudin 5 protein in bEnd.3 cells in the presence of LPS (scale bar: 10 μm). All data are presented as mean ± SD of five independent experiments. # p < 0.05, ## p < 0.01, ### p < 0.001 vs. indicated group, * p < 0.05, ** p < 0.01, *** p < 0.001 vs. LPS group; ns: no significant difference.