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. 2022 Mar 31;15(4):434. doi: 10.3390/ph15040434

Figure 6.

Figure 6

ZLN005 improves TGF-β1-induced renal tubular epithelial cell fibrosis partially depends on PGC-1α. (A) Western blotting was performed to determine expression of PGC-1α protein in siRNA-transfected HKC cells. (B) Quantitative analysis of PGC-1α protein levels in control siNC- or PGC-1α-siRNA-transfected HKC cells. Data are expressed as the mean ± SEM. (n = 3). ** p < 0.01. (C) The mRNA level of Fibronectin, α-SMA, Col1a1, Col1a4, TGFβ and Ppargc1α were measured in siNC- or PGC-1α-siRNA-transfected HKC cells after the corresponding α treatments. Data are expressed as the mean ± SEM. (n = 3~4). ** p < 0.01. Representative images of immunocytofluorescence staining of α-SMA (D) and Fibronectin (F) in siNC- or PGC-1α-siRNA-transfected HKC cells after the corresponding α treatments. Bar = 20 μm. Quantification of α-SMA (E) and Fibronectin (G) fluorescence intensity in each cell. Data are expressed as the mean ± SEM. (α-SMA: n = 21~35, Fibronectin: n = 15~25). * p < 0.05, ** p < 0.01. Level of Ppargc1α (H), α-SMA (I) and Fibronectin (J) mRNA were detected by quantitative RT-PCR in HKC cells after the corresponding α treatments. Data are expressed as the mean ± SEM. (n = 4). * p < 0.05, ** p < 0.01. ZLN005 is abbreviated to ZLN.