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. 2022 Apr 15;14(4):824. doi: 10.3390/v14040824

Figure 4.

Figure 4

RNAs levels and packaging efficiency. At 24 h post-transfection, (A) Total cellular RNA was extracted and purified. The amounts of viral RNA (FL, MS, and env) and cellular RNA (U6 and 7SL) within cells were determined by RT-qPCR. The copy numbers were normalised to that of cellular GAPDH mRNA. (B) Total RNA in virions was concentrated, and the intravirion levels of viral RNA (FL, MS, and env) and cellular RNA (U6 and 7SL) were determined by qPCR. (C) The p24 concentrations in virion-containing supernatants were determined in the presence or absence of 10% TritonX-100, using a p24-ELISA. (D) The viral RNA contents were calculated by the percentage of total RNA level in virions at 100 ng of p24 (RNA copies/100 ng p24 × 100) normalised to HIV ∆env alone. (E) The packaging efficiency was calculated by the total viral RNA in virions relative to total cellular RNA and normalised to HIV ∆env alone. Data represent the mean ± SD from the triplicate independent assay. *** p < 0.001; ** p < 0.01; * p < 0.1; ns, not significant using one-way ANOVA.