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. 2022 Apr 15;14(4):824. doi: 10.3390/v14040824

Figure 5.

Figure 5

CD81 tetraspanin organisation at the PM during HIV-1 release. HeLa or HeLa cells stably expressing ankyrin (EGFP) were transduced with VSV-G-pseudotyped HIV-1 GagmCherry. After 48 h, cells were harvested, and stained with Pacific Blue™ anti-human CD81. CD81 tetraspanin organisation was visualised under conventional confocal microscopy. (A) Non-transduced HeLa cells, (B) Transduced HeLa cells, (C) HeLa cells stably expressing Myr (+) AnkA32D3, (D) Transduced HeLa cells stably expressing Myr (+) AnkA32D3, (E) HeLa cells stably expressing Myr (+) AnkGAG1D4, (F) and transduced HeLa cells stably expressing Myr (+) AnkGAG1D4. Scale bar: 10 µm. The image shown in this figure is a representative data of several imaged fields. Blue, green and red represent CD81 tetraspanin, EGFP-tagged ankyrin, and GagmCherry, respectively.