Skip to main content
. 2022 Apr 12;14(8):1946. doi: 10.3390/cancers14081946

Figure 7.

Figure 7

Elevated MYC activity in the context of elevated SOX2 induces cell death. (A) Western blot analysis of nuclear extracts from i-SOX2/MYC-ER-ONS76 cells. Extracts were harvested after 48 h in the presence or absence of Dox and 4-OHT. (B) Proliferation of i-SOX2/MYC-ER-ONS76 cells as determined by MTT assay following 4 days of culture in the presence of Dox and 4-OHT at the indicated doses. (C) Proliferation of i-SOX2/MYC-ER-ONS76 and i-SOX2-ONS76 cells was determined by MTT assay following 4 days of culture in the presence or absence of 10 nM 4-OHT. (D) Photomicrographs of i-SOX2/MYC-ER-ONS76 cells after 4 days of culture in the presence or absence of Dox and 4-OHT. (E) Western blot analysis of cleaved PARP and cleaved Caspase-3 using whole cell extracts prepared from cells treated for 48 h in the presence or absence of Dox and 4-OHT at the concentrations indicated. N.S., not significant; * p < 0.05, ** p < 0.01, *** p < 0.001.