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. 2022 Apr 12;14(8):1946. doi: 10.3390/cancers14081946

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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SOX2 elevation downregulates MYC transcription. (A) Western blot analysis of MYC protein levels in i-SOX2-ONS76 cells treated with 100 ng/mL Dox for the indicated times. (B) RT-qPCR analysis of MYC mRNA levels in i-SOX2-ONS76 cells treated with 100 ng/mL Dox for the indicated times. Error bars represent standard deviations. (C) RT-qPCR analysis of MYC mRNA levels after 24 h of treatment at the indicated Dox dosage followed by 5 mg/mL actinomycin D treatment for the times indicated. MYC mRNA levels in control and Dox-treated cells were normalized to the 0 actinomycin D time point. (D) NRO-qPCR analysis of i-SOX2-ONS76 and i-SOX2-HCT116 cells. Cells were cultured in the indicated doses of Dox for 24 h before being subjected to NRO-qPCR analysis. Error bars represent standard deviation. ** p < 0.01, *** p < 0.001.