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. 2001 Jan;45(1):196–202. doi: 10.1128/AAC.45.1.196-202.2001

FIG. 1.

FIG. 1

Effects of subinhibitory concentrations of amoxicillin, gentamicin, and moxifloxacin on the expression of hla and α-toxin of S. aureus strains 8325-4 and DU 1090 in vitro. (A) Total RNAs of S. aureus strains 8325-4 (lanes 2 to 5) and DU 1090 (lane 6) were electrophoresed, blotted, and hybridized with an hla-specific probe in the absence of drugs (lane 2) or in the presence of amoxicillin (lane 3), gentamicin (lane 4), or moxifloxacin (lane 5). (B) Culture supernatants of S. aureus strains 8325-4 (lanes 2–5) and DU 1090 (lane 6) grown in the absence of drugs (lane 2) or in the presence of amoxicillin (lane 3), gentamicin (lane 4), or moxifloxacin (lane 5) were electrophoresed and blotted onto nitrocellulose, and α-toxin was detected with specific rabbit antibodies against α-toxin. Lane 1, 25 ng of purified α-toxin.