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Cleavage of purified α-toxin of S. aureus by human neutrophil elastase. (A) Quantitative determination of α-toxin cleavage by scanning of a Western blot. (B) Western blot of 100 ng of α-toxin (lane 1) pretreated with 0 ng (lane 2), 1 ng (lane 3), 10 ng (lane 4), 100 ng (lane 5), and 1,000 ng (lane 6) of neutrophil elastase or a mixture of 10 μg of α1-antitrypsin and 1 μg of neutrophil elastase (lane 7).
