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. 2022 Apr 22;23:319. doi: 10.1186/s12864-022-08541-5

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 7 — Analysis of mutations in samples sequenced on the GridION and the MiSeq: Consensus genomes produced by Genome Detective were uploaded to Nextclade and the results were analyzed. RUN116 was run on both platforms and the number and type of mutations detected by each platform was compared using a Wilcoxon rank sum test (Fig. A and C). A consensus file for all runs, for each platform, was produced and uploaded to Nextclade and a Wilcoxon rank sum test was performed to compare the number and type of mutations detected by both platforms (Fig. B and D). GridION samples are represented in yellow, whilst MiSeq samples are presented in green. Deletions, insertions, and substitutions are represented in pink, green, and blue, respectively. Statistical significance (Wilcoxon p tests) is represented by “*” (ns: non-significant, ***: p < 0.001, ****: p < 0.0001)