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. 2022 Apr 22;23:319. doi: 10.1186/s12864-022-08541-5

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 8 — Phylogenetic comparison between identical samples sequenced using both the GridION and MiSeq: A phylogenetic tree was created using IQTREE and visualized using FigTree for samples from Run116 sequenced on both the GridION and the MiSeq but classified in different clades by Nextclade. Only one of the 27 samples, represented in blue, clustered on the same branch. GridION genomes are annotated as ‘barcode*’, whilst MiSeq genomes are annotated as ‘K0*’