Table 2.
Comparison of the genome coverage and assigned clade for run116 samples on Nextclade
| Coverage (%) | Clade | |||
|---|---|---|---|---|
| Sample | GridION | MiSeq | GridION | MiSeq |
| K013400 | 72 | 92 | 20C | 20A |
| K013408 | 57 | 86 | 20H (Beta, V2) | 20C |
| K013410 | 70 | 90 | 20C | 20A |
| K013411 | 76 | 93 | 20H (Beta, V2) | 20A |
| K013415 | 63 | 91 | 20C | 20A |
| K013417 | 63 | 94 | 20C | 20A |
| K013418 | 62 | 94 | 20C | 20A |
| K013423 | 63 | 91 | 20C | 20A |
| K013425 | 60 | 93 | 20C | 20A |
| K013426 | 57 | 89 | 20C | 20A |
| K013429 | 64 | 95 | 20H (Beta, V2) | 20C |
| K013432 | 65 | 93 | 20C | 20A |
| K013433 | 50 | 94 | 20C | 20A |
| K013434 | 68 | 94 | 20C | 20A |
| K013437 | 35 | 92 | 20H (Beta, V2) | 20C |
| K013445 | 65 | 91 | 20C | 20A |
| K013447 | 92 | 98 | 20A | 20D |
| K013449 | 49 | 94 | 20C | 20A |
| K013450 | 68 | 97 | 20C | 20A |
| K013452 | 68 | 94 | 20C | 20A |
| K013454 | 56 | 90 | 20C | 20A |
| K013462 | 51 | 92 | 20C | 20A |
| K013465 | 60 | 94 | 20C | 20A |
| K013467 | 50 | 92 | 20C | 20A |
| K013470 | 72 | 89 | 20C | 20H (Beta, V2) |
| K013476 | 69 | 91 | 20C | 20H (Beta, V2) |
| Total | 20A | 1 | 20 | |
| 20C | 22 | 3 | ||
| 20D | 0 | 1 | ||
| 20H (Beta, V2) | 4 | 3 | ||
The table above highlights the 27 samples which were sequenced on both the MiSeq and the GridION but were classified in different clades by Nexclade. Clades identified by the GridION include 20A (n = 1), 20C (n = 22), and 20H (Beta, V2) (n = 4). Clades identified by the MiSeq include 20A (n = 20), 20C (n = 3), 20D (n = 1), and 20H (Beta, V2) (n = 3). There was also an overall higher genomic coverage for sequences from the MiSeq when compared to the GridION.