Table 3. Experimental Data and Their Interpretation.
| enzyme | X– | KMa [mM] | vmaxa [nmol/(min mg)] | kcatb,d (10–3/s) | kcat/KMb,d [1/(s M)] | ΔTSG°c,d (kJ/mol) | ref |
|---|---|---|---|---|---|---|---|
| AtHTMT1 | NCS– | 0.099 ± 0.020 | 43.6 ± 2.52 | 19.2 ± 1.11 | 193.9 ± 40.85 | 82.82 ± 0.14 | (14) |
| AtHTMT1 | Br– | 24.87 ± 2.785 | 11.4 ± 0.31 | 5.02 ± 0.14 | 0.202 ± 0.023 | 86.15 ± 0.07 | (14) |
| AtHTMT1 | Cl– | 145.2 ± 26.56 | 2.43 ± 0.12 | 1.07 ± 0.05 | 0.007 ± 0.001 | 89.98 ± 0.12 | (14) |
| PtHTMT | NCS– | 7.9 | — | 10.2 | 1.29 | 84.39 | (15) |
| PtHTMT | I– | 8.6 | — | 51.6 | 6.00 | 80.37 | (15) |
| PtHTMT | Br– | 72.8 | — | 1.04 | 0.0143 | 90.05 | (15) |
| PtHTMT | Cl– | 637.9 | — | 0.31 | 0.00048 | 93.05 | (15) |
| RsHTMT | NCS– | 0.04 | 185.185 | 89.5 | 2237.65 | 80.37 | (16) |
| RsHTMT | I– | 4.47 | 139.286 | 67.3 | 15.0608 | 81.09 | (16) |
| RsHTMT | Br– | 177.34 | 34.965 | 16.9 | 0.09530 | 84.58 | (16) |
| RsHTMT | Cl– | 1657.40 | 3.381 | 1.63 | 0.00099 | 90.46 | (16) |
| OsHol1 | NCS– | 0.15 | 0.966 | 0.42 | 2.91 | 92.29 | (17) |
| OsHol1 | I– | 0.07 | 5.190 | 2.27 | 34.13 | 88.12 | (17) |
| OsHol1 | Br– | 44.6 | 3.390 | 1.48 | 0.03 | 89.18 | (17) |
| OsHol1 | Cl– | no activity observed | — | — | — | (17) | |
a
Copied from the reference.
b
Calculated from published kinetic data when vmax is available; otherwise copied from the reference.
c
This work, calculated from experimental kinetic data.
d
Error range calculated directly from the error range stated for the data obtained from experiment.