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Regression analysis of PPi exchange activities based on the design matrix in Table 2. (a) Multivariate model for the activation free energy, ΔG‡kcat, and table of β coefficients with standard deviations, Student’s t-tests, and p-value probabilities. Symbols: diamonds = LeuRS, circles = LeuAC, empty symbols = no added leucine, red symbols = plus KF. Supplemental plots of the effects of [leucine] (b) and KF (c) clarify the interpretation of two-way interactions, as noted in the text.
