Figure 2.

Schematic diagram of methods for purifying iPSC samples. (A) LCST behavior of PDEGMA. iPSCs are cultured on the polymer at 37°C for 5 days and grow into colonies on the layer. By cooling the temperature to 22°C, iPSC colonies detach from the layer while differentiated cells remain on the layer. (B) After incubation with SpheriTech beads coated with affinity antibody, the target cells in a hetergenous cell suspension bind to the beads. Label-free target cells are eluted from the beads by trypsin and affinity antibodies still stay attached to the beads. (C) Compared with SpheriTech beads, antibodies or magnetic particles attached to the target cells may influence the cells for positive selection by MACS. HCS: heterogeneous cell suspension; TCs: target cells.