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. 2022 Mar 10;33(4):666–676. doi: 10.1021/acs.bioconjchem.2c00067

Figure 5.

Figure 5

Labeling of S. aureus with Chromeo488-strep–IgG. Bacteria were incubated with IgG and Chromeo488-strep (0.5 and 1 μM, respectively). (A) STED image collected under excitation at 488 nm and detection 495–550 nm using a depletion beam at 592 nm. The fluorescence signal is distributed on the bacterial wall. Scale bar 1 μm. (B,C) Magnified regions from the STED image. Scale bars 500 nm. Normalized intensity profiles measured across interesting domains on the bacterial wall are shown to the right of the corresponding magnified images. In the same intensity plots, the confocal profile is shown in comparison to the STED one (acquisition taken in a second sequential frame). A multi-peak fit (orange line) has been used to assess the dimension of the domains. Estimated widths are reported in each plot.