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Selective labeling of S. aureus with chromeo488-strep–IgG in a mixed culture with E. coli. (A) Transmitted light image of bacterial cells showing the different morphology of S. aureus (spherical) and E. coli (rod-shaped). The latter ones are highlighted in boxes 1, 2, and 3. Scale bar 5 μm. (B) Confocal image collected under excitation at 488 nm and detection 495–550 nm. Scale bar 5 μm. Interestingly, the fluorescence signal is absent on E. coli walls (boxes 1, 2, and 3 in B).
