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. 2022 Apr 5;144(15):6791–6801. doi: 10.1021/jacs.2c00274

Figure 5.

Figure 5

The active/deactive state of complex I assessed in co-reconstituted proteoliposomes. (A) The amounts of A and D complex I in a typical sample of as-prepared (AP) or deactivated (D) proteoliposomes were determined by sensitivity to derivatization with NEM (FET) or (B) by RET activity. (C) The proportion of A and D complex I determined in the DDM-solubilized enzyme. All measurements are normalized to the maximum rate for each set (CI:AOX = 35.5 μmol min–1 (mg CIout)−1, GlpD:CI = 0.618 μmol min–1 (mg CIout)−1, and CI:DQ = 14.3 μmol min–1 (mg CI)−1). All data are mean averages with error (± S.D.) values from three technical replicates.