Figure 6.

Comparison of catalysis by different species of complex I reconstituted into proteoliposomes. (A) Representative FET activities for proteoliposomes containing complex I from B. taurus (Bt), M. musculus (Mm), Y. lipolytica (Yl), and P. pastoris (Pp) in the presence and absence of 0.5 μg mL^–1 gramicidin. (B) Comparison between rates of NADH-linked ATP synthesis (S) and RET (R) in proteoliposomes. Rates are calculated per mg of outward-facing complex I. (C) Analysis of the proportion of active complex I in as-prepared proteoliposomes, determined by sensitivity of catalysis to NEM, relative to a DMSO vehicle control. All data are mean averages with error (± S.D.) values from quadruplicate technical replicates.