Figure 3.

Increased Angiotensin II (Ang II), AT2 receptor, and ACE2 levels and activity in a training volume-dependent manner: (A) Ang II levels in the soleus muscle; (B) AT1 and AT2 receptors mRNA levels; (C) angiotensin-converting enzyme 2 (ACE2) protein expression normalized for α-tubulin accompanied by its representative blot; (D) ACE2 activity. Groups: SC, sedentary control (n = 7); P1, swimming trained following protocol 1 (n = 7); P2, swimming trained following protocol 2 (n = 7). Each dot indicates a biological replication. Results are expressed as mean ± SEM. Statistical analysis: one-way ANOVA; significantly different vs. * SC, p < 0.05; ** SC, p < 0.01; **** SC, p < 0.0001; † P1, p < 0.05; ‡ P1, p < 0.01.