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. 2022 Apr 5;11(4):439. doi: 10.3390/pathogens11040439

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Comparison of the real-time RPA assay and qPCR for ASFV isolates and clinical specimens. The time to detection of the real-time RPA assay and qPCR were measured using11 ASFV isolates and clinical specimens collected from pigs challenged with ASFV (a) Volgograd-v (genotype II) or (b) Congo-v (genotype I). The time to a positive result for the tested specimens is expressed in seconds.