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. 2022 Apr 12;3(2):101292. doi: 10.1016/j.xpro.2022.101292

PCR cycling conditions

Step Temp Duration Cycles
Pre-activation 95°C 15 min 1
Denaturation 94°C 15 s 35
Annealing 64°C 30 s
Extension 72°C 30 s
Final extension 72°C 10 min 1