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. Author manuscript; available in PMC: 2023 Apr 13.
Published in final edited form as: Cell Host Microbe. 2022 Mar 21;30(4):530–544.e6. doi: 10.1016/j.chom.2022.02.017

Fig 3: Glut1 regulation is mediated by dectin-1/PKCδ pathway in neutrophils.

Fig 3:

(A) Schematic diagram of downstream signaling pathways of dectin-1. The diagram also shows different inhibitors or gene knockout mice used to sequentially block the signaling intermediates in dectin-1 signaling pathway. (B) BM neutrophils from WT or Card9−/− mice were stimulated with C. albicans (C. a) (MOI=0.2) or curdlan (Cur) (10 μg/ml) for 3 h with or without indicated inhibitors. Gene expression of Slc2a1 was measured by qPCR. (C-F) BM neutrophils from WT or Card9−/− mice were stimulated with C. a (MOI=1) or curdlan (100 μg/ml) in the presence or absence of indicated inhibitors. Phosphorylation of Glut1 was assessed by western blot at 30 min post-stimulation. Data pooled from at least 3 independent experiments (B-F) and representative images are shown. Statistical analysis by Two-way ANOVA (B). Data are represented as mean ± SEM (B). See also Fig S2.