Fig 5: Glut1 deficiency suppresses glycolysis in neutrophils during fungal stimulation.

(A) Glut1 expression in mouse peripheral blood neutrophils was measured by flow cytometry. (B) Glut1 and Glut3 protein levels in BM neutrophils were measured by western blot. (C) The number of neutrophils in blood and spleen of 6–7 weeks old male mice was assessed by flow cytometry. (D) PMN^WT and PMN^ΔGLUT1 mice were infected with C. albicans (C. a) for 24 h (n=4–6). Glucose uptake by kidney infiltrating neutrophils (GlucoseCy5⁺ cells) was detected by flow cytometry. (E-I) BM neutrophils from PMN^WT or PMN^ΔGLUT1 mice were stimulated with curdlan (Cur) (10 μg/ml) for 3 h. Cell pellets were subjected to metabolomics by untargeted high-resolution LC-HRMS. Data pooled from at least 3 independent experiments (A-I) and representative images and flow cytometry plots are shown. Statistical analysis by Student’s T test (C) or Two-way ANOVA (D and I). Data are represented as mean ± SEM (C, D, I). See also Fig S4, S5.