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. Author manuscript; available in PMC: 2023 Apr 13.
Published in final edited form as: Cell Host Microbe. 2022 Mar 21;30(4):530–544.e6. doi: 10.1016/j.chom.2022.02.017

Fig 7: Glut1 is critical for fungicidal function of human neutrophils.

Fig 7:

Healthy donor neutrophils (n=10) were stimulated with C. albicans (C. a) (MOI=0.2) for 3 h. (A) Gene expression of SLC2A1 was measured by qPCR. (B) Neutrophils isolated from healthy volunteers were stimulated with C. a (MOI=1) or curdlan (100 μg/ml) for 30 min. Glut1 phosphorylation was assessed by western blot. Representative image and ImageJ quantification of band intensities are shown. Healthy donor neutrophils were stimulated with C. a (MOI=1) in the presence or absence of Glut1 inhibitor (WZB117: 10 μM) for 1.5 h. (C) Glucose uptake by neutrophils was detected by flow cytometry, and (D) candidacidal activity of neutrophils was assessed by in vitro fungal killing assay. Statistical analysis by Student’s T test (A, D) and One-way ANOVA (B, C). Data are represented as mean ± SEM (A, B). See also Table S1.