Figure 3.

Huaier polysaccharide reduced PRV XJ5 adsorption and entry. The cells were treated according to the method described in Section 2.3.3. (A) Western blot showing changes in gB and actin expression. (B) The intensity band ratio of intracellular gB to actin. Data are presented as means from three independent statistical experiments. The intensities of protein bands were quantified using Image J. Significance was analyzed using one-tailed Student’s t-test. **** p < 0.0001. (C) The viral titers were evaluated through TCID₅₀ assay. (D) The numbers of cells infected with PRV were observed through IFA. (E) The virus DNA copies were observed through qRT-PCR after cells were washed with citric acid and PBS to remove uninternalized virus. ** p = 0.003, **** p < 0.0001. Data are shown as mean ± SD based on three independent experiments.