Figure 4.

Huaier polysaccharide attenuates PRV XJ5 adsorption. PK15 cells were treated according to the method described in Section 2.3.4. (A) At 24 h.p.i., PRV gB and actin protein expression were analyzed through the Western blot assay. (B) The intensity band ratio of intracellular gB to actin. The intensities of protein bands were quantified using Image J. (C) The viral titers were evaluated through TCID₅₀ assay. (D) Infected PRV XJ5 cells were observed through IFA. (E) The virus DNA copies were observed through qRT-PCR after cells were washed with PBS to remove unadsorbed virus. * p = 0.0219, **** p < 0.0001. Data are shown as mean ± SD based on three independent experiments.