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. 2022 Apr 1;14(4):745. doi: 10.3390/v14040745

Figure 5.

Figure 5

Huaier polysaccharide influences entry of pseudorabies virus in PK15 cells. The PK15 cells were treated according to the method described in Section 2.3.5. (A) At 24 h.p.i, PRV gB and actin protein expression were quantified through Western blot assay. (B) The relative intensity of intracellular gB to that of actin. Data are presented as the mean value from three independent statistical experiments. The relative intensities of protein were quantified using Image J. Significance was analyzed using the one-tailed Student’s t-test. (C) The viral titers were evaluated through TCID50 assay. (D) Immunofluorescence assay (IFA) for internalized virus was performed. (E) Intracellular viral DNA copies were analyzed by qRT-PCR after cells were washed with citric acid and PBS to remove uninternalized virus. *** p = 0.0001, **** p < 0.0001. Data are shown as mean ± SD based on three independent experiments.