Figure 6.

Huaier polysaccharide affects PRV XJ5 replication in PK15 cells. The PK15 cells were treated according to the method described in Section 2.3.6. At 4 and 6 h.p.i., the cells were collected to evaluate the expression of PRV gB and actin proteins through Western blotting (A,C). (B,D) The relative intensity of intracellular gB to that of actin. The relative intensities of protein were quantified using Image J. (E,F) PRV viral DNA copies were analyzed by qRT-PCR at 4 and 6 h.p.i. Significance was analyzed using the one-tailed Student’s t-test. NS, * p = 0.0299, ** p = 0.0017, *** p = 0.0001, **** p < 0.0001. Data are shown as mean ± SD based on three independent experiments.