Fig. 2.

TLR4 activation assay. Colourimetric response of HEKBlue cells to TLR4 activation at different dilutions of W3110, Raptor and ClearColi cells (a) or E. coli 0111:B4 endotoxin dilutions measured in endotoxin units (EU) per ml (b). All dilutions were represented in triplicate and mean, blank-adjusted values plotted, with error bars representing standard error of the mean. For A significance was determined using two-way ANOVA with Tukey’s multiple comparison test. Only significance for pairwise comparisons between samples, at each dilution are displayed. A full list of all comparisons is shown in Additional file 1: Table S5. For B significance was determined using one-way ANOVA with Tukey’s multiple comparison test. For both a and b ns P > 0.05, P** < 0.01, P*** < 0.001, P**** < 0.0001