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. 2022 Mar 23;12(4):488. doi: 10.3390/biom12040488

Figure 2.

Figure 2

In vitro differentiation of primary rat myocytes.Primary rat myocytes induced to differentiate for 1 to 18 days were decorated with antibodies against RyR1 to label the j-SR. At the beginning of differentiation, RyRs show a diffuse distribution in the SR. Starting from day 4 of differentiation, RyRs form clusters, and the SR progressively forms peripheral couplings and/or diads with the TT. Triads form during the following days, and at the end of differentiation, they acquire their transverse orientation and localize at the borders between the A and I bands of the sarcomeres.