Figure 1.

The effects of thiBPQ knockout on the TPP content, growth, and infection of ZW1. (A) The intracellular TPP levels of ZW1 and ZW1ΔTT were analyzed with a reporter plasmid. The higher the fluorescence intensity, the lower the TPP content. (B) Growth curves of ZW1 and ZW1ΔTT in Luria–Bertani broth (LB) medium. (C,D) Turbot were infected with ZW1, ZW1ΔTT, or ZW1ΔTTc, and bacterial recoveries from spleen (C) and kidney (D) were determined by plate count at different time points. Data are presented as means ± SD, n = 3. ** p < 0.01. Data marked with different letters have statistically significant differences (p < 0.05).