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. 2022 Apr 13;11(4):464. doi: 10.3390/pathogens11040464

Figure 5.

Figure 5

Thiamine uptake affects flagellar gene expression and bacterial motility through c-di-GMP. The gene expression of the fliF (A), flhB (B), and fliC (C) operons in ZW1, ZW1ΔTT, and ZW1ΔTT-adrA was measured by qRT-PCR with topA as an internal reference. For the convenience of comparison, the expression levels in ZW1 were normalized as 1. Data are presented as means ± SD, n = 3. Data marked with different letters have statistically significant differences (p < 0.05). (D) The motility of ZW1, ZW1ΔTT, and ZW1ΔTT-adrA on LB medium containing 0.3% (w/v) agar.