Fig. 3.

Chemical shift-based disorder analysis confirms that the human (A) and chicken (B) TRPV4-IDR are highly disordered throughout the entire protein sequence. (i-vii) Secondary chemical shifts calculated from the experimentally determined and predicted (using POTENCI, Nielsen and Mulder 2018) N, C’, C^α, C^β, H^α, H^β, H^N chemical shifts. (viii) Secondary structure prediction based on C^α, C^β, H^α chemical shifts using the SSP script (Marsh et al. 2006). Positive and negative values reflect α-helix and β-sheet propensities, respectively