Figure 7.

ARC attenuate LPS-induced phosphorylation of extracellular signal-regulated kinase (ERK1/2) and activation of NF-κB pathway in endothelial cells. (A) HMEC were stimulated with LPS (1 μg/mL) in the absence or presence of nanovesicles for 30 min, labeled with anti-p-ERK and analyzed by flow cytometry (one-way ANOVA followed by Bonferroni multiple comparisons test; * p < 0.05 vs. basal; # p < 0.05 between the labeled groups). HMEC were stimulated with LPS (1 μg/mL) in the absence or presence of nanoarchaeosomes (ARC) or liposomes (LIP) for 30 min (B) or 1 h (C) and cell extracts were analyzed by Western blot for p-ERK or IkBα, respectively. Representative images.