Table 2.
The two main anthocyanins in the profile extracted by different methods from three sample types and identified by ultra high liquid chromatography- electrospray ionization tandem mass spectrometry (UHPLC-ESI-MS). The pH of the extract, the compound name, formula, retention time and charge/mass ratio is presented.
| Method | Sample Type | pH | Compound Name | Formula | RT (Min) | m/z |
|---|---|---|---|---|---|---|
| Fresh | 3.02 | Pelargonidin Petunidin |
C15H11O5 C16H13O7 |
10.04 16.84 |
271.06 317.06 |
|
| Clf-Methanol (Solovchenko) (1) | Frozen | 3.65 | Pelargonidin Cyanidin |
C15H11O5 C15H11O6 |
10.03 16.84 |
271.06 287.05 |
| Freeze-dried | 3.024 | Pelargonidin Cyanidin |
C15H11O5 C15H11O6 |
10.04 16.83 |
271.06 287.05 |
|
| Fresh | 2.21 | Delphinidin Cyanidin |
C15H11O7 C15H11O6 |
12.98 16.82 |
303.05 287.05 |
|
| Methanol (Solovchenko) (2) | Frozen | 1.70 | Delphinidin Cyanidin |
C15H11O7 C15H11O6 |
12.98 16.81 |
303.05 287.05 |
| Freeze-dried | 1.06 | Delphinidin Pelargonidin |
C15H11O7 C15H11O5 |
12.99 10.04 |
303.05 271.06 |
|
| Fresh | 1.20 | Delphinidin Cyanidin-3-O-galactoside |
C15H11O7 C21H21O11 |
12.99 7.09 |
303.05 449.11 |
|
| Methanol (Lindoo) (3) | Frozen | 1.23 | Delphinidin Cyanidin-3-O-galactoside |
C15H11O7 C21H21O11 |
12.99 7.08 |
303.05 449.11 |
| Freeze-dried | 1.17 | Delphinidin Cyanidin-3-O-galactoside |
C15H11O7 C21H21O11 |
12.99 7.04 |
303.05 449.11 |
|
| Fresh | 1.73 | Delphinidin Malvidin-3-O-arabinoside |
C15H11O7 C22H23O11 |
12.97 7.38 |
303.05 463.12 |
|
| Clf-methanol (Neff) (4) | Frozen | 1.70 | Delphinidin Pelargonidin-3-O-sophoroside-5-O-glucoside |
C15H11O7 C33H41O20 |
12.98 8.82 |
303.05 757.22 |
| Freeze-dried | 1.63 | Delphinidin Pelargonidin |
C15H11O7 C15H11O5 |
12.96 10.08 |
303.05 271.06 |
|
| Fresh | 1.67 | Delphinidin Pelargonidin |
C15H11O7 C15H11O5 |
12.99 10.06 |
303.05 271.06 |
|
| pH differential (Lee) (5) | Frozen | 1.25 | Delphinidin Pelargonidin-3-O-sophoroside-5-O-glucoside |
C15H11O7 C33H41O20 |
12.99 8.83 |
303.05 757.22 |
| Freeze-dried | 1.26 | Delphinidin Pelargonidin-3-O-sophoroside-5-O-glucoside |
C15H11O7 C33H41O20 |
13 8.84 |
303.05 757.22 |
|
| Fresh | 1.63 | Delphinidin-3,5-O-diglucoside Delphinidin |
C27H31O17 C15H11O7 |
1.1 12.98 |
627.16 303.05 |
|
| Combined (Gauche) (6) | Frozen | 1.32 | Delphinidin Delphinidin-3,5-O-diglucoside |
C15H11O7 C27H31O17 |
12.98 1.11 |
303.05 627.16 |
| Freeze-dried | 1.30 | Delphinidin Delphinidin-3,5-O-diglucoside |
C15H11O7 C27H31O17 |
12.98 1.1 |
303.05 627.16 |
Numbers (superscript) in the brackets refer to the method number. Abbreviations: Six methods were tested: (1) and (2) methanol method by Solovchenko et al. [5] with and without chloroform respectively, (3) methanol method by Lindoo and Caldwell [6], (4) chloroform-methanol method by Neff & Chory [7], (5) pH differential method by Lee et al. [10], (pH 1.0), and (6) combination of methanol and pH differential methods by Gauche et al. [11], step 2, (pH 1.0).