Table 2.
Phenolic profile and content of aqueous acetone extracts obtained from Quince fruit (Cydonia oblonga Mill.) by LC-PDA-ESI-MS/MS.
| Compound | 60% | 65% | 70% | 75% | 80% | 85% | 90% | 95% | 100% |
|---|---|---|---|---|---|---|---|---|---|
| HYDROXYCINNAMIC ACIDS | |||||||||
| quinic acid | 487.06 ± 38.34 | 433.33 ± 60.18 | 511.54 ± 27.22 | 562.46 ± 27.32 | 394.73 ± 83.38 | 210.55 ± 30.31 | 128.83 ± 12.77 | 59.85 ± 17.85 | 69.70 ± 27.82 |
| chlorogenic acid | 87.32± 6.39 | 99.73 ± 8.70 | 105.57 ± 8.61 | 115.14 ± 15.34 | 114.56 ± 17.97 | 120.12 ± 10.68 | 112.83 ± 13.84 | 122.02 ± 10.59 | 17.20 ± 4.32 |
| caffeic acid | 0.15 ± 0.02 | 0.16 ± 0.02 | 0.22 ± 0.06 | 0.19 ± 0.03 | 0.20 ± 0.01 | 0.23 ± 0.02 | 0.19 ± 0.02 | 0.28 ± 0.02 | ND |
| HYDROXYBENZOIC ACIDS | |||||||||
| shikimic acid | 9.73 ± 1.39 | 11.54 ± 1.34 | 11.58 ± 1.05 | 12.08 ± 1.21 | 15.02 ± 2.61 | 20.48 ± 1.13 | 22.70 ± 5.86 | 23.44 ± 2.94 | 3.05 ± 41.26 |
| gallic acid | 0.03 ± 0.00 | 0.07 ± 0.04 | 0.01 ± 0.00 | 0.03 ± 0.00 | 0.02 ± 0.00 | 0.03 ± 0.01 | 0.03 ± 0.01 | ND | ND |
| protocatechuic acid | 0.03 ± 0.01 | 0.03 ± 0.00 | 0.02 ± 0.01 | 0.04 ± 0.01 | 0.03 ± 0.00 | 0.03 ± 0.01 | 0.01 ± 0.00 | 0.02 ± 0.00 | 0.01 ± 0.00 |
| 2,5-dihydroxybenzoic acid | 0.24 ± 0.07 | 0.16 ± 0.05 | 0.24 ± 0.08 | 0.33 ± 0.03 | 0.30 ± 0.02 | 0.35 ± 0.01 | 0.22 ± 0.01 | 0.23 ± 0.03 | 0.02 ± 0.00 |
| vanillic acid | 0.05 ± 0.02 | 0.03 ± 0.01 | 0.06 ± 0.01 | 0.06 ± 0.01 | 0.13 ± 0.04 | 0.06 ± 0.01 | 0.06 ± 0.01 | 0.07 ± 0.01 | TR |
| t-cinnamic acid | 0.05 ± 0.00 | 0.11 ± 0.01 | 0.07 ± 0.00 | 0.12 ± 0.00 | 0.17 ± 0.07 | 0.09 ± 0.00 | 0.13 ± 0.02 | 0.27 ± 0.04 | 0.03 ± 0.00 |
| benzoic acid | 0.61 ± 0.05 | 0.87 ± 0.09 | 0.83 ± 0.05 | 0.93 ± 0.2 | 0.94 ± 0.11 | 0.67 ± 0.10 | 0.87 ± 0.14 | 0.77 ± 0.21 | 0.68 ± 0.12 |
| 2-hydroxybenzoic acid | 0.10 ± 0.00 | 0.10 ± 0.00 | 0.09 ± 0.00 | 0.10 ± 0.00 | 0.15 ± 0.02 | 0.09 ± 0.00 | 0.08 ± 0.01 | 0.09 ± 0.00 | 0.06 ± 0.03 |
| syringic acid | TR | 0.02 ± 0.00 | 0.03 ± 0.01 | TR | 0.04 ± 0.01 | 0.07 ± 0.02 | 0.07 ± 0.00 | 0.17 ± 0.02 | ND |
| 4-hydroxybenzoic acid | 0.30 ± 0.04 | 0.37 ± 0.10 | 0.42 ± 0.03 | 0.45 ± 0.03 | 0.47 ± 0.18 | 0.45 ± 0.06 | 0.47 ± 0.10 | 0.50 ± 0.04 | 0.13 ± 0.00 |
| coumaric acid | 0.04 ± 0.01 | 0.07 ± 0.01 | 0.07 ± 0.02 | 0.07 ± 0.01 | 0.08 ± 0.02 | 0.08 ± 0.02 | 0.10 ± 0.02 | 0.09 ± 0.00 | 0.01 ± 0.00 |
| ferulic acid | ND | 0.02 ± 0.00 | 0.01 ± 0.00 | 0.02 ± 0.00 | 0.01 ± 0.00 | 0.01 ± 0.00 | 0.01 ± 0.00 | 0.03 ± 0.01 | TR |
| FLAVAN-3-OLS | |||||||||
| procyanidin b2 | 259.59 ± 40.01 | 217.44 ± 45.98 | 184.56 ± 8.1 | 138.14 ± 14.1 | 150.29 ± 49.42 | 58.27 ± 19.27 | 28.36 ± 0.48 | 4.56 ± 1.72 | 5.24 ± 3.97 |
| epicatechin | 3.91 ± 0.60 | 3.27 ± 0.69 | 2.78 ± 0.12 | 2.08 ± 0.21 | 2.26 ± 0.74 | 0.87 ± 0.29 | 0.42 ± 0.00 | 0.06 ± 0.02 | TR |
| catechin | 0.19 ± 0.02 | 0.16 ± 0.04 | 0.13 ± 0.02 | 0.13 ± 0.02 | 0.11 ± 0.03 | 0.07 ± 0.02 | 0.04 ± 0.01 | 0.02 ± 0.00 | 0.02 ± 0.00 |
| FLAVONOLS | |||||||||
| rutin | 274.14 ± 43.18 | 281.96 ± 29.78 | 276.17 ± 61.09 | 232.54 ± 18.86 | 233.75 ± 36.43 | 219.77 ± 22.35 | 171.68 ± 19.77 | 214.74 ± 25.24 | 17.91 ± 8.68 |
| kaempferol-3-o-glucoside | 4.16 ± 0.35 | 4.49 ± 0.23 | 4.58 ± 0.19 | 4.08 ± 0.80 | 4.27 ± 0.65 | 4.35 ± 0.67 | 3.94 ± 0.65 | 3.93 ± 0.19 | 0.71 ± 0.23 |
| quercetin | 0.25 ± 0.01 | 0.27 ± 0.03 | 0.24 ± 0.08 | 0.34 ± 0.04 | 0.42 ± 0.10 | 0.41 ± 0.03 | 0.76 ± 0.00 | 1.03 ± 0.07 | 0.04 ± 0.00 |
| kaempferol | 0.02 ± 0.00 | 0.02 ± 0.00 | 0.13 ± 0.00 | TR | TR | TR | TR | TR | 0.17 ± 0.07 |
| taxifolin | 0.1 ± 0.00 | TR | TR | TR | TR | TR | TR | TR | TR |
| FLAVANONES | |||||||||
| naringenin | ND | TR | TR | ND | TR | TR | TR | TR | ND |
| FLAVONES | |||||||||
| acacetin | TR | 0.01 ± 0.00 | 0.01 ± 0.00 | TR | TR | TR | TR | TR | TR |
| DIHYDROCHALCONES | |||||||||
| neohesperidin | 0.18 ± 0.07 | 0.21 ± 0.07 | 0.23 ± 0.05 | 0.33 ± 0.03 | 0.20 ± 0.03 | 0.28 ± 0.07 | 0.25 ± 0.06 | 0.25 ± 0.06 | 0.07 ± 0.03 |
| phloretin | TR | TR | ND | ND | TR | TR | TR | TR | ND |