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Cell-free synthesis of Ctx and LT. Single subunits and co-expressed subunits of Ctx (A) and LT (B) were synthesized in a CHO lysate. Total protein yields of de novo synthesized protein were analyzed by liquid scintillation for the translation mixture (TM). Standard deviations were calculated from triplicate analysis. Autoradiographs depicting 14C-labeled protein bands after a synthesis in CHO lysate for Ctx (C) and LT (D). A no-template control (NTC) was used as a negative control.
