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Synthesis of CtxAamb mutants and labeling using orthogonal systems. (A) Schematic overview of the orthogonal translation. (B) In-gel fluorescence of CtxAamb mutants labeled with DyLight-632 phosphine after orthogonal translation in an optimized CHO system. (C) In-gel fluorescence and autoradiography of co-expressed multimer after the incorporation of AzF and subsequent labeling with DyLight-632 phosphine.
