Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Apr 22;21:103. doi: 10.1186/s12943-022-01524-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 5 — Regulation of CDK6-PI3K axis on ABCB1-mediated drug resistance. Knockout of cdk6 gene in KB-C2 with CRISPR/Cas9 technique induced attenuation of ABCB1-mediated drug resistance. It promoted AS in the pre-mRNA expressing ABCB1, generating differential expression of the transcripts encoding functional ABCB1 protein or truncated abnormal peptides, further decreased the level of functional ABCB1 transporters. With the deficiency of cdk6 in KB-C2 cells, PI3K 110α and 110β expression were downregulated, which induced AS of ABCB1 pre-mRNA and led to inhibition of ABCB1 expression and reversal of ABCB1-mediated MDR, inhibiting cell division and promoting cell apoptosis. A series of signaling pathways critical for malignancies were downregulated in the KB-C2-k.o.cdk6 cell population