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. 2022 Mar 24;11(4):504. doi: 10.3390/biology11040504

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Age-related decline in bone marrow B cells. (A) Flow cytometric analysis of bone marrow cells from control and Miz-1^ΔPOZ mice. Cells were analyzed with antibodies for B220 and CD19. Cells are pre-gated for debris and doublet exclusion. Percentages in dot plots are given for the respective gates. (B) Frequency of B cells (B220⁺CD19⁺) from bone marrow of control and Miz-1^ΔPOZ mice. (C) Quantification B cells (B220⁺CD19⁺) from bone marrow of control and Miz-1^ΔPOZ mice, corrected to the number of living cells. One point represents one mouse. Data are expressed as mean + SD (n = 5). Student’s unpaired t-test: ** p < 0.01 *** p < 0.001, **** p < 0.0001. For (A), plots are representative of at least five independent experiments.