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. 2022 Mar 24;11(4):504. doi: 10.3390/biology11040504

Figure 2.

Figure 2

Decrease in immature and recirculating B cell subsets in the bone marrow with age. (A) Flow cytometric analysis of bone marrow cells from control and Miz-1ΔPOZ mice. Cells were analyzed with antibodies for B220 and IgM. Cells are pre-gated for debris and doublet exclusion. Percentages in dot plots are given for the respective gates and were corrected to the number living cells. (B) Frequency of early B cells (B220+IgM−). (C) Frequency of B220highIgM− B cells in the bone marrow. (D) Frequency of immature B cells (B220+CD19+CD93+CD43−IgM+) in the bone marrow of control and Miz-1ΔPOZ mice at indicated ages. (E) Frequency of recirculating B cells (B220+CD19+CD93−CD43−IgM+) in the bone marrow of control and Miz-1ΔPOZ mice at indicated ages. One point represents one mouse. Data are expressed as mean + SD (n ≥ 3). Student’s unpaired t-test: * p < 0.05, ** p < 0.01 *** p < 0.001, **** p < 0.0001. For (A), plots are representative of at least five independent experiments.