. 2022 Apr 14;14(8):1647. doi: 10.3390/nu14081647

Table 2.

Findings of fecal microbiota transplant (FMT) in relation to myasthenia gravis (MG) using mice.

	Findings
Animal study on ^a FMT	Using an open field test, 4 weeks after ^a FMT [97] - Colonization of ^b GF mice with ^c MMb resulted in an impaired locomotion ability. - The effect may be reversed by colonizing ^b GF mice with both ^c MMb and ^d HMb. - Levels of ^e TNF-α, ^f IFN-γ, and ^g IL-10 in both serum and intestinal tissue were much greater in the ^c MMb group than the ^d HMb group. The co-administration of ^d HMb could reverse the effects of increased cytokines in ^c MMb mice. 16S rRNA gene sequencing, 4 weeks after ^a FMT [97] - Distinct microbial communities of MG patients were reproducible in ^a FMT, ^c MMb, and ^d HMb mice. - A total of 98 ^h OTUs belonging to Firmicutes (49/98), Bacteroides (34/98), and Actinobacteria (3/98) were identified. - A total of 54 of the 98 differential ^h OTUs between ^c MMb and ^d HMb were reversed in the ⁱ CMb group - A total opf 16 of the 54 reversed ^h OTUs belonging to Lachnospiraceae (7 ^h OTU), Bacteroidaceae (4 ^h OTU), and Ruminococcaceae (2 ^h OTU) were associated with impaired locomotion ability and the interference of fecal metabolomics.

Findings

Animal study on ^a FMT

Using an open field test, 4 weeks after ^a FMT [97]

-
Colonization of ^b GF mice with ^c MMb resulted in an impaired locomotion ability.
-
The effect may be reversed by colonizing ^b GF mice with both ^c MMb and ^d HMb.
-
Levels of ^e TNF-α, ^f IFN-γ, and ^g IL-10 in both serum and intestinal tissue were much greater in the ^c MMb group than the ^d HMb group. The co-administration of ^d HMb could reverse the effects of increased cytokines in ^c MMb mice.

16S rRNA gene sequencing, 4 weeks after ^a FMT [97]

-
Distinct microbial communities of MG patients were reproducible in ^a FMT, ^c MMb, and ^d HMb mice.
-
A total of 98 ^h OTUs belonging to Firmicutes (49/98), Bacteroides (34/98), and Actinobacteria (3/98) were identified.
-
A total of 54 of the 98 differential ^h OTUs between ^c MMb and ^d HMb were reversed in the ⁱ CMb group
-
A total opf 16 of the 54 reversed ^h OTUs belonging to Lachnospiraceae (7 ^h OTU), Bacteroidaceae (4 ^h OTU), and Ruminococcaceae (2 ^h OTU) were associated with impaired locomotion ability and the interference of fecal metabolomics.

^a FMT: fecal microbiota transplant; ^b GF: germ-free; ^c MMb: myasthenia gravis microbiome; ^d HMb: healthy microbiota; ^e TNF-α: tumor necrosis factor alpha; ^f IFN-γ: interferon gamma; ^g IL-10: interleukin 10; ^h OTU: Operational Taxonomic Unit; ⁱ CMb: both healthy microbiota and myasthenia gravis microbiome.