Figure 2.

HPV L2 stabilizes the γ-secretase complex. (A) HeLa cells were infected as in Figure 1A, and lysates were collected in lysis buffer containing 1% DDM. The PS1 subunit of γ-secretase was immunoprecipitated with anti-PS1 and samples were analyzed by Western blotting for HPV and the indicated γ-secretase subunit as in Figure 1A. Similar results were obtained in three independent experiments. (B) As in (A), except anti-APH1 was used for immunoprecipitation. Similar results were obtained in seven independent experiments. (C) As in (A), except cells were infected with HPV16 (WT) or HPV5 PsV. Similar results were obtained in two independent experiments. (D) As in (A), except cells were lysed in buffer containing 1% of the indicated detergent. Similar results were obtained in two independent experiments. (E) As in (A), except cells were harvested at the indicated time after addition of PsV. (F) As in (A), except cells were infected with complete PsV (HPV16) or L1-only HPV16 PsV. Similar results were obtained in an additional independent experiment using anti-APH1.