Table 3.

Available bioinformatics tools for sgRNA designing.

Tool Name	Link	Reference
CRISPR-GE	http://skl.scau.edu.cn/	[94]
CRISPRdirect	http://crispr.dbcls.jp/	[98]
CRISPR-P	http://cbi.hzau.edu.cn/crispr/	[99]
CRISPResso	http://crispresso.rocks/	[100]
E-CRISP	http://www.e-crisp.org/E-CRISP/	[101]
BreakingCas	http://bioinfogp.cnb.csic.es/tools/breakingcas/index.php	[102]
CRISPR-DO	http://cistrome.org/crispr/	[103]
CRISPOR	http://crispor.tefor.net/	[104]
CT-Finder	http://bioinfolab.miamioh.edu/ct-finder/	[105]
sgRNACas9	http://www.biootools.com/	[106]
CRISPR design	http://crispr.mit.edu/	[107]
Cas9 design	http://Cas9.cbi.pku.edu.cn/	[95,108]
Cas-Designer	http://www.rgenome.net/cas-designer/	[109]
CGAT	http://cbc.gdcb.iastate.edu/cgat/	[108]
Cas-OFFinder	http://www.rgenome.net/cas-offinder/	[110]
CCTop	http://crispr.cos.uni-heidelberg.de/	[111]
ProtospacerWB	http://www.protospacer.com/	[112]
SSC	http://crispr.dfci.harvard.edu/SSC/	[113]
CRISPR multi targeter	http://www.multicrispr.net/	[114]
MAGeCK	https://sourceforge.net/p/mageck/wiki/Home/	[115]
GT-Scan	http://gt-scan.braembl.org.au/gt-scan/	[116]
GuideScan	http://www.guidescan.com/	[101]
CrisprGE*	http://crdd.osdd.net/servers/crisprge/	[117]

‘–’: information unavailable.